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Cell Culture, Micro- & Molecular Biology
Simple Dilution
Calculate stock volume needed using C₁V₁ = C₂V₂
C₁ × V₁ = C₂ × V₂  →  V₁ = (C₂ × V₂) / C₁
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Serial Dilution
Generate a stepwise dilution series with a constant dilution factor
Molarity Calculator
Calculate molarity, mass, or volume — leave exactly one field blank
n = m / M  |  c = n / V  →  c = m / (M × V)
g/mol
Leave exactly one field blank — that value will be calculated.
Solution Preparation from Solid
How much solid do I need to prepare a solution of given molarity?
m = c × V × M
% Solutions
w/v, v/v and w/w concentrations — leave exactly one field blank
g
mL
%
PCR Master Mix Calculator
Calculate component volumes for multiple reactions
Component Final conc. Stock conc. Vol/rxn (µL) Total (µL)
Total master mix volume: µL ( rxn incl. overage)
DNA/RNA Concentration (NanoDrop)
Calculate concentration and purity from absorbance readings
Cell Count & Seeding
Hemocytometer cell density and seeding volume calculation
Hemocytometer
Neubauer improved: Count cells in 4 corner squares (each 1×1 mm). Formula: (n / squares) × dilution × 10,000

Seeding / Passage
Cell Seeding
Calculate stock volume, target density or final volume — leave exactly one field blank
C₁ × V₁ = C₂ × V₂  →  cells in = cells out
Leave exactly one field blank — that value will be calculated.
cells/mL
cells/mL
Centrifuge: RCF ↔ RPM
Convert between relative centrifugal force and revolutions per minute
RCF = 1.118 × 10⁻⁵ × r [cm] × n [rpm]²
Leave exactly one field blank — that value will be calculated.
Unit Converter
Convert concentration and volume units
Concentration

Volume

Mass